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KMID : 0378019670100070111
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New Medical Journal
1967 Volume.10 No. 7 p.111 ~ p.120
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Effect of Fracture and Sex Hormones on Metabolism of glucose-C^(14) in Mouse Bone
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Abstract
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One hundered and sixty male mice, weighing approximately 25gm, were devided into eight groups, each consisting of 20 mice.
In the first four groups, 25gm, of various sex hormones were injected subcutaneously in vivo into each mouse for 3 days. The left femur of each mouse was then fractured by manual clasis, 2 days after this, the mice were slaughtered and the both femora of each mouse were freed of soft tissue attachment, and dreid, then pulverized and homogenized in a fluid incubation media containing glucose-C14.
In the later half in vitro groups, the left femur was first fractured and two days later the mice were staughtered and the both femora were freed of soft tissue and then immersed in the same amount of sex hormones for 24 hours
The femora were next dried, pulverized and homogenized in the same incubation media. With the homogenates thus obtained oxidative metabolism of glucose was observed by means of CO, production rate and of RGD.
Following observations were made.
1) Oxidative metabaosm of glucose is accelerated in the fractured bone. & bothin vivo and in vitro groups
2) In both the fractured & non-fractured bones, oxidative metobolism of glucose is accelerated when sex hormones are administered; the rate of acceJration being androgen, estrogen, and progesterone, in that order.
3) Oxidative metabolism of glucose in the non-farctured bones is accelerated. with the administration of sex hormones but not as much as the fractured bones.
4) The fact that glucose metabolism is also accelerated when sex hormones are admministered, suggests, in the author´s opinion, that these hormones act directly on bone.
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KEYWORD
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